Skip to main content
Addgene

pJK59-ATG17-GFP
(Plasmid #44175)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44175 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pJK59
  • Backbone size w/o insert (bp) 5756
  • Total vector size (bp) 7307
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Atg17
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1551
  • Mutation
    none
  • Entrez Gene
    ATG17 (a.k.a. YLR423C, APG17)
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer internal to gene
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The plasmid with GFP was obtained from Will Prinz.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Constructed by amplifying Atg17 with 300bp 5' of the start site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK59-ATG17-GFP was a gift from James Hurley (Addgene plasmid # 44175 ; http://n2t.net/addgene:44175 ; RRID:Addgene_44175)

  • For your References section:

    Architecture of the Atg17 complex as a scaffold for autophagosome biogenesis. Ragusa MJ, Stanley RE, Hurley JH. Cell. 2012 Dec 21;151(7):1501-12. doi: 10.1016/j.cell.2012.11.028. Epub 2012 Dec 6. 10.1016/j.cell.2012.11.028 PubMed 23219485
Related items:
Commonly requested with: