pInducer20
(Plasmid #44012)

• Purpose: (Empty Backbone) Tet-inducible lentiviral vector for ORF expression
• Depositing Lab: Stephen Elledge
• Publication: Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi: 10.1073/pnas.1019736108. Epub 2011 Feb 9.

Backbone

• Vector backbone: pHAGE-TRE
• Backbone manufacturer: Elledge
• Backbone size (bp): 11791
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Neomycin (select with G418)
• Tag / Fusion Protein:
  • HA (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): ccdB Survival
• Growth instructions: pInducer20’s should be grown in LB with 50 ug/ml of ampicillin and 30 ug/ml of chloramphenicol.
• Copy number: Unknown

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: LNCX_primer

Resource Information

• Articles Citing this Plasmid:
  • 154 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pHAGE-TRE vector originally based on pHAGE-CAGS-W vector (Dr. Richard Mulligan, Harvard Institute of Medicine, Boston)