pInducer20
(Plasmid #44012)

• Purpose: (Empty Backbone) Tet-inducible lentiviral vector for ORF expression
• Depositing Lab: Stephen Elledge
• Publication: Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi: 10.1073/pnas.1019736108. Epub 2011 Feb 9.

Backbone

• Vector backbone: pHAGE-TRE
• Backbone manufacturer: Elledge
• Backbone size (bp): 11791
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Neomycin (select with G418)
• Tag / Fusion Protein:
  • HA (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): ccdB Survival
• Growth instructions: pInducer20’s should be grown in LB with 50 ug/ml of ampicillin and 30 ug/ml of chloramphenicol.
• Copy number: Unknown

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: LNCX_primer

Resource Information

• Articles Citing this Plasmid:
  • 154 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pHAGE-TRE vector originally based on pHAGE-CAGS-W vector (Dr. Richard Mulligan, Harvard Institute of Medicine, Boston)

Information for Cloning Grade DNA (Catalog # 44012-DNA.cg) ( Back to top)

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $105 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here