pIM1708-surT-PsurP-agaN-pNW33N
(Plasmid #44009)

• Depositing Lab: Ichiro Matsumura
• Publication: Blanchard et al Appl Microbiol Biotechnol. 2014 May 2.

Backbone

• Vector backbone: pNW33N
• Backbone manufacturer: Bacillus Genetic Stock Center
• Backbone size w/o insert (bp): 4174
• Total vector size (bp): 7680
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): INVaF'
• Growth instructions: May require up to 24 hours of growth for sufficient colonies when plated (and for preps)
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: surT-PsurP-agaN
• Alt name: alpha galactosidase
• Alt name: Geobacillus stearothermophilus transcription antiterminator
• Species: Geobacillus stearothermophilus NUB3621
• Insert Size (bp): 3506
• Mutation: Deleted EcoRI site (synonymous change, C to T at position 967) in surT region
• GenBank ID: AF130985.1 U34873.1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: PstI (not destroyed)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

IMPORTANT NOTE: Even though there is a partial AmpR gene noted on the map, this plasmid is NOT known to confer Amp resistance. The CAT gene is the first ORF (270-920) and the Matsumura lab has only used Chloramphenicol for selection. The backbone, pNW33N, was not engineered by the depositing lab and the sequence they present here is theoretical. Though there are discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor, the plasmid should function as described.