MM39
(Bacterial strain
#42894)
-
Depositing Lab
-
Publication
-
Sequence Information
-
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Bacterial Strain | 42894 | Bacteria in agar stab | 1 | $85 |
Backbone
-
Vector backbonegenome
Growth in Bacteria
-
Bacterial Resistance(s)Streptomycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)MM39
-
Growth instructionsSelection using 10-15 micrograms/mL (ug/mL)
-
Copy numberLow Copy
Cloning Information
- Cloning method Unknown
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
selection using 10-15 micrograms/mL strep.
It is MalE deficient, which enables maltose complementation. For the assay, a particular construct is transformed into MM39 and grown on maltose minimal media to confirm proper membrane orientation and integration.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
MM39 was a gift from Bryan Berger (Addgene plasmid # 42894) -
For your References section:
Identifying Key Juxtamembrane Interactions in Cell Membranes Using AraTM. Su PC, Berger BW. J Biol Chem. 2012 Jul 22. 10.1074/jbc.M112.396895 PubMed 22822084