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Addgene

pAquaN1
(Plasmid #42888)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42888 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pECFP-N1
  • Backbone manufacturer
    Clontech
  • Total vector size (bp) 4700
  • Modifications to backbone
    None
  • Vector type
    Mammalian Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Aquamarine
  • Alt name
    ECFP-T66S-H149D
  • Species
    A. victoria
  • Mutation
    T66S and H149G

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAquaN1 was a gift from Fabienne Merola (Addgene plasmid # 42888 ; http://n2t.net/addgene:42888 ; RRID:Addgene_42888)

  • For your References section:

    Minimum set of mutations needed to optimize cyan fluorescent proteins for live cell imaging. Erard M, Fredj A, Pasquier H, Beltolngar DB, Bousmah Y, Derrien V, Vincent P, Merola F. Mol Biosyst. 2013 Feb 2;9(2):258-67. doi: 10.1039/c2mb25303h. Epub 2012 Nov 28. 10.1039/c2mb25303h PubMed 23192565
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