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Addgene

pCas9
(Plasmid #42876)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42876 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pACYC184
  • Backbone size w/o insert (bp) 4244
  • Total vector size (bp) 9326
  • Vector type
    Bacterial Expression, CRISPR ; E.coli

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    pCas9 is resistant to chloramphenicol (25ug/ml).
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    tracr/Cas9

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The HME63 strain is available from the from the Court Lab:
https://redrecombineering.ncifcrf.gov/strains--plasmids.html

For more information on Marraffini Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/marraffini/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCas9 was a gift from Luciano Marraffini (Addgene plasmid # 42876 ; http://n2t.net/addgene:42876 ; RRID:Addgene_42876)

  • For your References section:

    RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Jiang W, Bikard D, Cox D, Zhang F, Marraffini LA. Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2508. 10.1038/nbt.2508 PubMed 23360965
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