pCDNA6A-EGFR WT
(Plasmid #42665)

• Depositing Lab: Mien-Chie Hung
• Publication: Hsu et al J Biol Chem. 2007 Apr 6;282(14):10432-40. Epub 2007 Feb 5.

Backbone

• Vector backbone: pcDNA6/myc-His A
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5100
• Vector type: Mammalian Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: EGFR
• Alt name: epidermal growth factor receptor
• Species: H. sapiens (human)
• Insert Size (bp): 1200
• Entrez Gene: EGFR (a.k.a. ERBB, ERBB1, ERRP, HER1, NISBD2, NNCIS, PIG61, mENA)
• Tags / Fusion Proteins:
  • Myc (C terminal on backbone)
  • 6xHis (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (unknown if destroyed)
• 3′ cloning site: XbaI (unknown if destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Articles Citing this Plasmid:
  • 13 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry