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Addgene

ZNF668-Flag
(Plasmid #42636)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42636 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p3xFLAG-CMV-10
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 6400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ZNF668
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1860
  • GenBank ID
    NM_024706.3
  • Entrez Gene
    ZNF668
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer CMV Forward
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ZNF668-Flag was a gift from Shiaw-Yih Lin (Addgene plasmid # 42636 ; http://n2t.net/addgene:42636 ; RRID:Addgene_42636)

  • For your References section:

    ZNF668 functions as a tumor suppressor by regulating p53 stability and function in breast cancer. Hu R, Peng G, Dai H, Breuer EK, Stemke-Hale K, Li K, Gonzalez-Angulo AM, Mills GB, Lin SY. Cancer Res. 2011 Oct 15;71(20):6524-34. doi: 10.1158/0008-5472.CAN-11-0853. Epub 2011 Aug 18. 10.1158/0008-5472.CAN-11-0853 PubMed 21852383
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