pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)
(Plasmid #42335)

• Purpose: A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.
• Depositing Lab: Feng Zhang
• Publication: Cong et al Science. 2013 Jan 3.

Backbone

• Vector backbone: pUC ori vector
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: humanized S. pyogenes Cas9 (D10A) nickase
• Alt name: SpCas9n (D10A)
• Alt name: hSpCas9n (D10A)
• Insert Size (bp): 4272
• Mutation: D10A nickase-converting mutation
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Resource Information

• Supplemental Documents:
  • pX335 annotated Genbank file
• Articles Citing this Plasmid:
  • 186 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note, Addgene's quality control sequencing has found a few discrepancies with the depositor's full sequence. The depositing lab is aware of these discrepancies, and they are not thought to affect plasmid function.

For more information on Zhang Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/zhang/

Information for Cloning Grade DNA (Catalog # 42335-DNA.cg) ( Back to top)

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $105 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here