pMHBAD14
(Plasmid #42304)

• Depositing Lab: Hideo Iwai
• Publication: Oeemig et al FEBS Lett. 2009 May 6;583(9):1451-6. doi: 10.1016/j.febslet.2009.03.058. Epub 2009 Apr 1.

Backbone

• Vector backbone: pBAD
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4786
• Total vector size (bp): 4909
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: NpuDnaE C-intein
• Alt name: C-intein of native split NpuDnaE
• Alt name: split intein
• Species: Nostoc punctiforme
• Insert Size (bp): 123
• Promoter: araBAD
• Tag / Fusion Protein:
  • GB1-H6 (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: pBAD Forward
• 3′ sequencing primer: pBAD Reverse

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There are 2 mismatches between Addgene's quality control and depositor's reference sequence. These changes are in backbone region and should not affect function.