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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 16084 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTRE-tight
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 2540
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namecaspase-3 (p12) :: nz
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Alt namecaspase-3
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SpeciesH. sapiens (human)
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Insert Size (bp)477
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Entrez GeneCASP3 (a.k.a. CPP32, CPP32B, SCA-1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer LNCX (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bycaspase-3 from MGC full length cDNA clone ID 4419175.
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
caspase-3 (p12) fused to the antiparallel leucine-zipper domain NZ (ALKKELQANKKELAQLKWELQALKKELAQ). For listed sequence, 356-664bp = caspase3(p12); 728-820bp = NZ.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTRE-Tight caspase-3 (p12)::nz [TU#817] was a gift from Martin Chalfie (Addgene plasmid # 16084 ; http://n2t.net/addgene:16084 ; RRID:Addgene_16084) -
For your References section:
Targeted cell killing by reconstituted caspases. Chelur DS, Chalfie M. Proc Natl Acad Sci U S A. 2007 Feb 13. 104(7):2283-8. 10.1073/pnas.0610877104 PubMed 17283333