Tol2-zTRAP
(Plasmid #42236)

• Depositing Lab: Stephen L Johnson
• Publication: Tryon et al Genesis. 2012 Dec 19. doi: 10.1002/dvg.22363.

Backbone

• Vector backbone: pT2KXIG
• Backbone size w/o insert (bp): 6170
• Total vector size (bp): 7574

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10/P3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EGFP-Rpl10a
• Species: D. rerio (zebrafish)
• Insert Size (bp): 1404
• GenBank ID: NM_199636.1
• Entrez Gene: rpl10a (a.k.a. wu:fb94f08, zgc:73082, zgc:86881)
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: ClaI (not destroyed)
• 5′ sequencing primer: GCAGGAAACGTGAGCAGAG

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
• Industry Terms:
  • Not Available to Industry