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PurposeTimeSTAMP method for tagging and visualization of newly synthesized proteins. TimeSTAMPa cassette used for for low abundance proteins or proteins with slow ongoing synthesis rates
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 42235 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCS6
- Total vector size (bp) 8172
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePSD95
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Tag
/ Fusion Protein
- TSa-HA (C terminal on insert)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS6-mPSD95a-TSa-HA was a gift from Roger Tsien (Addgene plasmid # 42235 ; http://n2t.net/addgene:42235 ; RRID:Addgene_42235) -
For your References section:
TimeSTAMP tagging of newly synthesized proteins. Lin MZ, Tsien RY. Curr Protoc Protein Sci. 2010 Feb;Chapter 26:Unit 26.5. doi: 10.1002/0471140864.ps2605s59. 10.1002/0471140864.ps2605s59 PubMed 20155731
Map uploaded by the depositor.
