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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 42110 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFLAG-CMV
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Backbone manufacturerSigma
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSec31a
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SpeciesH. sapiens (human)
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Insert Size (bp)3700
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Entrez GeneSEC31A (a.k.a. ABP125, ABP130, HPBKS, HSPC275, HSPC334, NEDSOSB, SEC31L1)
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FLAG-Sec31a #891#342 was a gift from Ayano Satoh (Addgene plasmid # 42110 ; http://n2t.net/addgene:42110 ; RRID:Addgene_42110) -
For your References section:
CK2 phosphorylates Sec31 and regulates ER-To-Golgi trafficking. Koreishi M, Yu S, Oda M, Honjo Y, Satoh A. PLoS One. 2013;8(1):e54382. doi: 10.1371/journal.pone.0054382. Epub 2013 Jan 18. 10.1371/journal.pone.0054382 PubMed 23349870