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PurposeRetroviral expression of DsRed in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 41943 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneMSCV
- Backbone size w/o insert (bp) 7556
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Vector typeMammalian Expression, Retroviral
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Selectable markersIRES d4EGFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDsRed
- Promoter TRE
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer pLXSN-5 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
4 hour half life EGFP
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MSCV-CMV-DsRed-IRES-d4EGFP was a gift from Stephen Elledge (Addgene plasmid # 41943 ; http://n2t.net/addgene:41943 ; RRID:Addgene_41943) -
For your References section:
Global protein stability profiling in mammalian cells. Yen HC, Xu Q, Chou DM, Zhao Z, Elledge SJ. Science. 2008 Nov 7;322(5903):918-23. doi: 10.1126/science.1160489. 10.1126/science.1160489 PubMed 18988847
