pAAH1
(Plasmid #41825)

• Purpose: (Empty Backbone) Used as a PCR template for tagging yeast proteins with the DHFR tag (c-terminal histag) by homologous recombination and selection for hygromycin resistance.
• Depositing Lab: Melissa Moore
• Publication: Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

Backbone

• Vector backbone: pAED4
• Backbone manufacturer: Paul Matsudaira lab
• Backbone size (bp): 3322
• Modifications to backbone: Contains the gene for the E. coli dihydrofolate reductase (DHFR) with a c-terminal histag and the hygromycin resistance cassette from pAG32Made by removing the hygromycin resistance cassette from pAG32 (Euroscarf) at the BamHI and SacI sites and ligation into pAED4-eDHFR (L. W. Miller, Y. Cai, M. P. Sheetz, V. W. Cornish, Nat. Methods 2, 255 (2005)).
• Vector type: Bacterial Expression, Yeast Expression ; PCR template
• Promoter: T7, TEF
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Cloning Information

• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Resource Information

• A portion of this plasmid was derived from a plasmid made by: We received pAED4 from another lab, but did not sign an MTA. We received the DHFR gene from Virginia Cornish.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry