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H9 pMalLP
(Plasmid #41811)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41811 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMalLP
  • Backbone size w/o insert (bp) 6700
  • Total vector size (bp) 7000
  • Modifications to backbone
    The vector is a variant of pMalp2x, with a modified MCS and an N-terminal 3C protease cleavage site.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    H9 Super-2
  • Alt name
    H9
  • Alt name
    Super-2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    429
  • Entrez Gene
    IL2 (a.k.a. IL-2, TCGF, lymphokine)
  • Promoter pTac
  • Tags / Fusion Proteins
    • Maltose Binding Protein (N terminal on backbone)
    • 3C protease site (N terminal on backbone)
    • 6x His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer Mal-E
  • 3′ sequencing primer M13-65
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This is an e. coli periplasmic expression vector.

H9 has the following mutations compared to wildtype IL-2: L80F, R81F, L85V, I86V, I92F

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    H9 pMalLP was a gift from Chris Garcia (Addgene plasmid # 41811 ; http://n2t.net/addgene:41811 ; RRID:Addgene_41811)

  • For your References section:

    Exploiting a natural conformational switch to engineer an interleukin-2 'superkine'. Levin AM, Bates DL, Ring AM, Krieg C, Lin JT, Su L, Moraga I, Raeber ME, Bowman GR, Novick P, Pande VS, Fathman CG, Boyman O, Garcia KC. Nature. 2012 Mar 25;484(7395):529-33. doi: 10.1038/nature10975. 10.1038/nature10975 PubMed 22446627
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