IL2 pMalLP
(Plasmid #41808)

• Depositing Lab: Chris Garcia
• Publication: Levin et al Nature. 2012 Mar 25;484(7395):529-33. doi: 10.1038/nature10975.

Backbone

• Vector backbone: pMalLP
• Backbone size w/o insert (bp): 6700
• Total vector size (bp): 7000
• Modifications to backbone: The vector is a variant of pMalp2x, with a modified MCS and an N-terminal 3C protease cleavage site.
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: IL2
• Alt name: Interleukin 2
• Species: H. sapiens (human)
• Insert Size (bp): 429
• Entrez Gene: IL2 (a.k.a. IL-2, TCGF, lymphokine)
• Promoter: pTac
• Tags / Fusion Proteins:
  • Maltose Binding Protein (N terminal on backbone)
  • 3C protease site (N terminal on backbone)
  • 6x His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: Mal-E
• 3′ sequencing primer: M13-65

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is an e. coli periplasmic expression vector.