pcDNA3-N-I-N-CAAX
(Plasmid #41686)

• Purpose: Expresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to Dronpa145N at C terminus and at N terminus, for optogenetic activation
• Depositing Lab: Michael Lin
• Publication: Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi: 10.1126/science.1226854.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5370
• Total vector size (bp): 7425
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Dronpa145N-IntersectinDH-Dronpa145N-CAAX
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 2055
• Mutation: K145N on Dronpa
• GenBank ID:
• Promoter: CMV
• Tag / Fusion Protein:
  • CAAX box (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: Xho1 (not destroyed)
• 5′ sequencing primer: XpressFWD: TATGGCTAGCATGACTGGT
• 3′ sequencing primer: SP6: ATTTAGGTGACACTATAG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: We synthesized the Dronpa and intersectin fragments from oligos.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry