pRS414-GPD-6xHis-Hsp82
(Plasmid
#41188)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 41188 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS414
- Backbone size w/o insert (bp) 5700
- Total vector size (bp) 7800
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Modifications to backbonemultiple cloning site altered to remove some restriction sites.
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Vector typeYeast Expression
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameS. cerevisiae Hsp82
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Alt nameHsp90
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)2130
- Promoter GPD
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Tag
/ Fusion Protein
- 6xHis (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer GPD forward
- 3′ sequencing primer CYC reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRS414-GPD-6xHis-Hsp82 was a gift from Dan Bolon (Addgene plasmid # 41188 ; http://n2t.net/addgene:41188 ; RRID:Addgene_41188) -
For your References section:
Dimerization of Hsp90 is required for in vivo function. Design and analysis of monomers and dimers. Wayne N, Bolon DN. J Biol Chem. 2007 Nov 30;282(48):35386-95. Epub 2007 Oct 1. 10.1074/jbc.M703844200 PubMed 17908693
Map uploaded by the depositor.
