pEGFP Cep164 (Nigg CW324)
(Plasmid #41149)

• Depositing Lab: Erich Nigg
• Publication: Graser et al J Cell Biol. 2007 Oct 22;179(2):321-30.

Backbone

• Vector backbone: pCJW204 (modified pEGFP-N3)
• Backbone manufacturer: Modified from Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 9100
• Modifications to backbone: FseI- and NotI-site-containing linker (AAT TCG GCC GGC CTA GCG GCC GCT GCA) inserted between EcoRI and PstI sites.
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cep164
• Alt name: centrosomal protein 164kDa
• Alt name: NPHP15
• Alt name: KIAA1052
• Species: H. sapiens (human)
• Insert Size (bp): 4400
• GenBank ID: NM_014956.4 AB028975
• Entrez Gene: CEP164 (a.k.a. NPHP15)
• Promoter: CMV
• Tag / Fusion Protein:
  • eGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: FseI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EGFP-N; SV40-pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Human Cep164 cDNA from clone KIAA1052 (Kazusa DNA Research Institute).
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PCR was used to amplify a full-length human Cep164 cDNA from clone KIAA1052 (Kazusa DNA Research Institute).