pRcCMV Cep164 (Nigg CW325)
(Plasmid #41148)

• Depositing Lab: Erich Nigg
• Publication: Graser et al J Cell Biol. 2007 Oct 22;179(2):321-30.

Backbone

• Vector backbone: pCJW206 (modified pEGFP-C1)
• Backbone manufacturer: Modified from Clontech
• Backbone size w/o insert (bp): 4110
• Total vector size (bp): 8500
• Modifications to backbone: FseI- and NotI-site-containing linker (AAT TCG GCC GGC CTA GCG GCC GCT GCA) inserted between EcoRI and PstI sites. Myc-tag-encoding fragment (aag ctt gct tac att tgc ttc tga cac aac tgt gtt cac tag caa cct caa aca gac acc atg gag cag aag ctg atc tcc gag gag gac ctg aac atg aat tc) inserted between HindIII and EcoRI sites. GFP-encoding sequence removed by excision with BspEI and AgeI and religation
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Cep164
• Alt name: centrosomal protein 164kDa
• Alt name: NPHP15
• Alt name: KIAA1052
• Species: H. sapiens (human)
• Insert Size (bp): 4400
• GenBank ID: NM_014956.4 AB028975
• Entrez Gene: CEP164 (a.k.a. NPHP15)
• Promoter: CMV
• Tag / Fusion Protein:
  • Myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: FseI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40-pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Human Cep164 cDNA from clone KIAA1052 (Kazusa DNA Research Institute).
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PCR was used to amplify a full-length human Cep164 cDNA from clone KIAA1052 (Kazusa DNA Research Institute). The cDNA was subcloned into a mammalian expression vector providing a myc epitope tag and the construct was verified by sequencing.