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BBa_J72113-BBa_J72152
(Plasmid #40784)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40784 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p15a, CamR
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Phagemid + GFP

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For detailed composition information, look up the backbone (BBa_J72113) and insert (BBa_J72152) on the Registry of Standard Biological Parts (partsregistry.org)

There is a G insert at 5860 of depositor's seq, in between the "phagemid" and GFP, T301C, T459C, these do not effect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BBa_J72113-BBa_J72152 was a gift from Christopher Anderson (Addgene plasmid # 40784 ; http://n2t.net/addgene:40784 ; RRID:Addgene_40784)

  • For your References section:

    Scalable plasmid transfer using engineered P1-based phagemids. Kittleson JT, DeLoache W, Cheng HY, Anderson JC. ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. Epub 2012 Aug 30. 10.1021/sb300054p PubMed 23656280
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