NNMT
(Plasmid #40734)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Cheryl Arrowsmith
• Publication: Toronto SGC Q1 Q2 2012 (unpublished)

Backbone

• Vector backbone: pET28a-LIC
• Backbone manufacturer: SGC
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: NNMT
• Alt name: nicotinamide N-methyltransferase
• Alt name: PDB:2IIP
• Species: H. sapiens (human)
• Insert Size (bp): 978
• Mutation: K100A:E101A:E103A
• GenBank ID: GI:66933018
• Entrez Gene: NNMT
• Tag / Fusion Protein:
  • His Thrombin (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

http://www.thesgc.org/structures/2IIP/