2C::tdTomato Reporter
(Plasmid #40281)

• Depositing Lab: Samuel Pfaff
• Publication: Macfarlan et al Nature. 2012 Jul 5;487(7405):57-63.

Backbone

• Vector backbone: pcDNA Hygro
• Backbone manufacturer: Invitrogen/Life Tech
• Backbone size w/o insert (bp): 6400
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 2C Promoter (2-cell stage promoter)
• Species: M. musculus (mouse)
• Insert Size (bp): 730
• Tag / Fusion Protein:
  • tdTomato

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Mlu1 (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: Amp-R
• 3′ sequencing primer: BGH-rev

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 13 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The CMV promoter from the parent pcDNA plasmid was removed with MluI and HindIII, leaving approx. 6400kb backbone. The 2C promoter was put in its place (730bp) using the same sites. Hence, the full plasmid is 7129bp. The promoter is active shortly after fertilization of mouse embryos to about the 8-cell stage. It is also on in a subset of MESCs & miPSCs.