pcDNA3-Clover
(Plasmid #40259)

• Purpose: GFP with robust performance in protein fusions and useful as FRET donor for mRuby2 in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap
• Depositing Lab: Michael Lin
• Publication: Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
• Citations: 40 Articles

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5410
• Total vector size (bp): 6127
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Clover GFP
• Species: Synthetic; Aequorea victoria
• Insert Size (bp): 717
• Promoter: CMV IE

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH Rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Clover is the brightest green fluorescent protein (GFP) characterized to date, with robust performance in protein fusions. Also, it is useful as a FRET donor to mRuby2 in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap.