pExodus CMV.Trex2
(Plasmid #40210)

• Purpose: Mammalian expression of codon-optimized Trex2.
• Depositing Lab: Andrew Scharenberg
• Publication: Certo et al Nat Methods. 2012 Oct;9(10):973-5. doi: 10.1038/nmeth.2177. Epub 2012 Sep 2.

Backbone

• Vector backbone: pUC
• Backbone manufacturer: In-house
• Backbone size w/o insert (bp): 4389
• Total vector size (bp): 5100
• Modifications to backbone: Multiple cloning site, CMV and T7 promoters
• Vector type: Mammalian Expression, Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Three prime repair exonuclease 2
• Alt name: Trex2
• Species: M. musculus (mouse)
• Insert Size (bp): 711
• Entrez Gene: Trex2
• Promoter: CMV and T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nhe1 (not destroyed)
• 3′ cloning site: EcoR1 (not destroyed)
• 5′ sequencing primer: T7 FP
• 3′ sequencing primer: BGH RP

Resource Information

• Addgene Notes:
  • Plasmid 40210 restriction digest
• A portion of this plasmid was derived from a plasmid made by: We received the Trex2 ORF-containing construct from the laboratory of Daniel Stetson, University of Washington
• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry