pFLLFLN
(Plasmid #40030)

• Depositing Lab: Liqun Luo
• Publication: Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Mar 27.

Backbone

• Vector backbone: Unknown
• Backbone size w/o insert (bp): 3500
• Total vector size (bp): 5489
• Vector type: Mammalian Expression, Bacterial Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: FRT-Lox5171-Lox2272-FRT-loxP
• Promoter: None

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: see sequence
• 3′ sequencing primer: see sequence

Gene/Insert 2

• Gene/Insert name: Neo
• Alt name: neomycin phosphotransferase
• Alt name: neomycin resistance gene
• Promoter: SV40

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: see sequence
• 3′ sequencing primer: see sequence

Resource Information

• Supplemental Documents:
  • GenBank_file

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Additional versions of this plasmid are available from Addgene: pLN and pFLN.