pSCC2
(Plasmid #39993)

• Depositing Lab: Paul Modrich
• Publication: Cheng et al J Biol Chem. 1984 Sep 25;259(18):11571-5.

Backbone

• Vector backbone: pKC30
• Backbone manufacturer: n/a
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 5200
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): JC4588 lambda+
• Growth instructions: Induce expression by transfer to 37˚C.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: EcoRI endonuclease & EcoRI methylase
• Species: E. coli
• Insert Size (bp): 2210
• Mutation: none
• GenBank ID: J01675.1
• Promoter: n/a
• Tag / Fusion Protein:
  • none

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HpaI (unknown if destroyed)
• 3′ cloning site: HpaI (unknown if destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pKC30 plasmid derivation is described in Nature (1981) 292, 128-132.

Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at or contact our distributors if you have any questions.