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Addgene

pAraGFP
(Plasmid #39548)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 39548 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD18
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eGFP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer ATG CCA TAG CAT TTT TAT CC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please see the Addgene verified sequence to see the exact GFP sequence. Note that these differences compared to the depositor's sequence do not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAraGFP was a gift from Bryan Berger (Addgene plasmid # 39548 ; http://n2t.net/addgene:39548 ; RRID:Addgene_39548)

  • For your References section:

    Identifying Key Juxtamembrane Interactions in Cell Membranes Using AraTM. Su PC, Berger BW. J Biol Chem. 2012 Jul 22. 10.1074/jbc.M112.396895 PubMed 22822084
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