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Addgene

pMG207
(Plasmid #39273)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 39273 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKSS
  • Backbone manufacturer
    Peter Kast
  • Vector type
    E coli Gene Targeting
  • Selectable markers
    Neomycin (select with G418) ; Neo/KanR is selected for after recombination

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Use this plasmid for homologous recombination and strain construction in E. coli. See article and Author's map for details.

Addgene's quality control sequencing results found a silent mutation at bp# 5965 when compared to the full plasmid sequence provided by the depositing laboratory. This difference does not affect the dsbA protein sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMG207 was a gift from Peter Kast (Addgene plasmid # 39273 ; http://n2t.net/addgene:39273 ; RRID:Addgene_39273)

  • For your References section:

    Strategy for chromosomal gene targeting in RecA-deficient Escherichia coli strains. Gamper M, Kast P. Biotechniques. 2005 Mar;38(3):405-8. 10.2144/05383ST03 PubMed 15786807
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