pet26-MBP-ELIC
(Plasmid
#39239)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 39239 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepet26
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Backbone manufacturerNovagen
- Total vector size (bp) 7478
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)BL21/de3
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameEriwinia chrysanthemi - pentameric ligand gated ion channel
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Alt nameELIC
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SpeciesEriwinia chrisanthemi
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Insert Size (bp)971
- Promoter T7
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Tags
/ Fusion Proteins
- His-10 (N terminal on backbone)
- MBP (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer MBP-Seq Primer
- 3′ sequencing primer T7 term (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For protein purification, grow until OD600 of 1.6-1.8. Induction with 0.3mM IPTG and expression at 20°C.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pet26-MBP-ELIC was a gift from Raimund Dutzler (Addgene plasmid # 39239 ; http://n2t.net/addgene:39239 ; RRID:Addgene_39239) -
For your References section:
Structure of a potentially open state of a proton-activated pentameric ligand-gated ion channel. Hilf RJ, Dutzler R. Nature. 2009 Jan 1. 457(7225):115-8. 10.1038/nature07461 PubMed 18987630
