pCEP4L-Erk1
(Plasmid
#39225)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 39225 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCEP4
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 10600
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Vector typeMammalian Expression ; episomal
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameErk1
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SpeciesH. sapiens (human)
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Insert Size (bp)1100
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Entrez GeneMAPK3 (a.k.a. ERK-1, ERK1, ERT2, HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, PRKM3, p44-ERK1, p44-MAPK)
- Promoter CMV
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCEP4L-Erk1 was a gift from Melanie Cobb (Addgene plasmid # 39225 ; http://n2t.net/addgene:39225 ; RRID:Addgene_39225) -
For your References section:
A requirement for extracellular signal-regulated kinase (ERK) function in the activation of AP-1 by Ha-Ras, phorbol 12-myristate 13-acetate, and serum. Frost JA, Geppert TD, Cobb MH, Feramisco JR. Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):3844-8. 10.1073/pnas.91.9.3844 PubMed 8170999