PCTK1
(Plasmid #39053)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Nicola Burgess-Brown
• Publication: SGC+year (unpublished)

Backbone

• Vector backbone: pNIC-CH
• Backbone manufacturer: SGC
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Mach1
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: PCTK1
• Alt name: PCTAIRE protein kinase 1 [isoform a; tv1]
• Alt name: PDB:3MTL
• Species: H. sapiens (human)
• Insert Size (bp): 981
• Mutation: Activation loop mutant S319D
• Entrez Gene: CDK16 (a.k.a. PCTAIRE, PCTAIRE1, PCTGAIRE, PCTK1)
• Tag / Fusion Protein:
  • His6 (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

http://www.thesgc.org/structures/3MTL/