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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 38772 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP (modified)
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Backbone manufacturerClontech
- Backbone size (bp) 4151
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Modifications to backboneEnhanced CFP (mammalian codon optimized) with monomerizing A206K mutation
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
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Tag
/ Fusion Protein
- mECFP (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Unknown
- 3′ sequencing primer EXFP-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid carrying monomeric (A206K) ECFP for C-terminal tagging
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmECFP-1 was a gift from Benjamin Glick (Addgene plasmid # 38772 ; http://n2t.net/addgene:38772 ; RRID:Addgene_38772)
Map uploaded by the depositor.
