Phi80 JQ929581
(Plasmid #38170)

• Depositing Lab: Drew Endy
• Publication: Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 21.

Backbone

• Vector backbone: Phi80
• Vector type: Synthetic Biology ; Integration vector

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): pir2+
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: mKate2
• Species: Synthetic
• Insert Size (bp): 850

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: EGFP-N
• 3′ sequencing primer: EGFP-C

Gene/Insert 2

• Gene/Insert name: Superfolder GFP
• Species: Synthetic
• Insert Size (bp): 812

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This construct has attB and attP sites (BP).

To integrate on your favorite E.coli strain, transform in strain containing the pAH123 plasmid (Haldimann et al., J of Bacteriology 2001). Contains a R6K origin of replication, will only replicate in pir2+ strains.