ECFP-Bcr in pCCL-cppt178-MNDU3
(Plasmid #38156)

• Depositing Lab: Nora Heisterkamp
• Publication: Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. Epub 2006 Nov 20.

Backbone

• Vector backbone: pCCL-cppt178-MNDU3
• Backbone manufacturer: Don Kohn
• Backbone size w/o insert (bp): 6600
• Total vector size (bp): 10900
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Bcr
• Species: H. sapiens (human)
• Insert Size (bp): 4300
• GenBank ID:
• Entrez Gene: BCR (a.k.a. ALL, BCR1, CML, D22S11, D22S662, PHL)
• Tag / Fusion Protein:
  • ECFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Age I (destroyed during cloning)
• 3′ cloning site: Kpn I (not destroyed)
• 5′ sequencing primer: EGFP-C

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Constructed by Jess Cunnick. The construct was made in two steps. First a 5' 1.7 kb BamHIxSstI fragment and a 3' SstIxKpnI fragment from two BCR plasmids were ligated into pECFP-C1 digested with Bgl II x KpnI. ECFP-Bcr was removed from the plasmid by digestion with Age I x KpnI and ligated into pCCL0cppt178-MNDU3-X3.