pPK719
(Plasmid #38149)

• Depositing Lab: Patricia Kuwabara
• Publication: Kuwabara unpublished plasmids (unpublished)

Backbone

• Vector backbone: pBluescript KSII(+)
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 6461
• Vector type: Worm Expression ; recombineering

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: cbr-unc-119::FRT*::galk::FRT*
• Alt name: rescue construct for unc-119(ed3) mutants
• Alt name: cbr-unc-119
• Species: C. elegans (nematode)
• Entrez Gene: Cbr-unc-119 (a.k.a. CBG18291)
• Promoter: unc-119

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: M13pUC-fwd

Resource Information

• Supplemental Documents:
  • pPK719 supplemental information
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid carries a complementary cassette consisting of a genomic copy of the C. briggsae unc-119 gene and the selectable galK marker flanked by FRT* sites in the presumptive 3’ UTR of the Cbr-unc-119 gene.

The 3.6 kb Cbr-unc-119::FRT*::galk::FRT* cassette can be targeted by recombineering to insert between nts 281:282 in the pCC1FOS fosmid vector backbone. In other words, this cassette can be readily inserted in all fosmids contained in the C. elegans library developed by Don Moerman and colleagues. We have tested the construct in fosmid recombineering and have shown that it rescues unc-119(ed3) mutants.