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Addgene

pXCX.CMV.tTA
(Plasmid #38056)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 38056 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pXCXCMV
  • Total vector size (bp) 8715
  • Vector type
    Mammalian Expression, Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    tTA
  • Alt name
    tet transactivator
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Adenoviral plasmid for overexpression of tet transactivator (tTA) from CMV promoter

Addgene's sequencing result identified an 82bp insert at bp# 699 when compared to the full plasmid sequence provided by the depositing laboratory. This difference does not affect the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pXCX.CMV.tTA was a gift from James Uney (Addgene plasmid # 38056 ; http://n2t.net/addgene:38056 ; RRID:Addgene_38056)

  • For your References section:

    Increased utility in the CNS of a powerful neuron-specific tetracycline-regulatable adenoviral system developed using a post-transcriptional enhancer. Lee YB, Cosgrave AS, Glover CP, Bienemann A, Heywood D, Hobson RJ, Uney JB. J Gene Med. 2005 May;7(5):576-83. 10.1002/jgm.694 PubMed 15580589
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