pPEP-TEV cNup98 (1-498)
(Plasmid #38037)

• Depositing Lab: Roderick Lim
• Publication: Kowalczyk et al Nat Nanotechnol. 2011 Jun 19;6(7):433-8. doi: 10.1038/nnano.2011.88.

Backbone

• Vector backbone: pPEP-TEV
• Backbone size w/o insert (bp): 2968
• Total vector size (bp): 4460
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Nucleoporin 98
• Alt name: Nup98
• Species: H. sapiens (human)
• Insert Size (bp): 1515
• Mutation: amino acids 1-498
• Entrez Gene: NUP98 (a.k.a. ADIR2, NUP196, NUP96, Nup98-96)
• Promoter: T7
• Tag / Fusion Protein:
  • His-tag, 3Cysteine (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CGCGGATCCTGTTGCTGTTTTAACAAATCATTTGGAACA
• 3′ sequencing primer: CCGGAATTCTTAAGAGTCTCCAAAAGG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pPEP-TEV empty plasmid was obtained from Prof. H. Herrmann and used to derive our plasmid (pPEP-TEV/cNup98)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

see also:
Strelkov, S. V., Kreplak, L., Herrmann, H. & Aebi, U. (2004). Methods Cell Biol.78, 25–43.