pAAV-CAG-Arch-GFP
(Plasmid #37810)

• Depositing Lab: Edward Boyden
• Publication: Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

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Backbone

• Vector backbone: AAV with CAG promoter
• Backbone manufacturer: Scott Sternson
• Backbone size w/o insert (bp): 4722
• Total vector size (bp): 6228
• Vector type: Mammalian Expression, AAV ; Adeno-associated virus

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl3
• Growth instructions: Use recombinase-free E. coli (Stbl3, XL-10, Sure2 and DH5a et.al), grow at 30C
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Arch
• Alt name: archaerhodopsin-3
• Alt name: H. sodomense archaerhodopsin-3
• Alt name: Arch-GFP
• Species: H. sodomense
• Insert Size (bp): 1506
• Mutation: N/A
• Promoter: CAG
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: tcggcttctggcgtgtgac
• 3′ sequencing primer: aaagagacagcaaccagg

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PLEASE CONTACT ED BOYDEN () FOR DETAILS ON THIS REAGENT AND FURTHER REAGENTS IN THIS LINE.