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Addgene

pGEN-mSmoA1
(Plasmid #37674)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37674 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEG
  • Modifications to backbone
    EGFP removed from pEGFP-C1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Smo
  • Species
    M. musculus (mouse)
  • Mutation
    W539L
  • Entrez Gene
    Smo (a.k.a. E130215L21Rik, Smoh, bnb, smoothened)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-Fwd
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene NGS results identified a D54N variant compared to the NCBI reference [EDL13766.1].

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEN-mSmoA1 was a gift from Philip Beachy (Addgene plasmid # 37674 ; http://n2t.net/addgene:37674 ; RRID:Addgene_37674)

  • For your References section:

    Effects of oncogenic mutations in Smoothened and Patched can be reversed by cyclopamine. Taipale J, Chen JK, Cooper MK, Wang B, Mann RK, Milenkovic L, Scott MP, Beachy PA. Nature. 2000 Aug 31;406(6799):1005-9. 10.1038/35023008 PubMed 10984056
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