pCEFLmGFP-Gli2
(Plasmid #37672)

• Depositing Lab: Philip Beachy
• Publication: Kim et al Proc Natl Acad Sci U S A. 2009 Dec 22;106(51):21666-71. Epub 2009 Dec 8.

Backbone

• Vector backbone: pCEFL
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Gli2
• Species: M. musculus (mouse)
• Entrez Gene: Gli2
• Promoter: EF1a
• Tag / Fusion Protein:
  • mGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: EF1a-Fwd
• 3′ sequencing primer: BGH-Rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pCEFL was provided by J. Silvio Gutkind (National Institutes of Health).
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

monomeric GFP was constructed by introducing the monomeric mutation, A206K, to EGFP.