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pMDR1-1202
(Plasmid #37627)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37627 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL2 Basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5598
  • Total vector size (bp) 6918
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    JM109
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MDR1 promoter
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1320
  • Entrez Gene
    ABCB1 (a.k.a. ABC20, CD243, CLCS, GP170, MDR1, P-GP, PGY1, p-170)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xma I (not destroyed)
  • 3′ cloning site Nhe I (destroyed during cloning)
  • 5′ sequencing primer GLprimer1
  • 3′ sequencing primer GLprimer2
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that this plasmid forms dimers (see diagnostic digest linked above).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMDR1-1202 was a gift from Kathleen Scotto (Addgene plasmid # 37627 ; http://n2t.net/addgene:37627 ; RRID:Addgene_37627)

  • For your References section:

    Transcriptional activation of the MDR1 gene by UV irradiation. Role of NF-Y and Sp1. Hu Z, Jin S, Scotto KW. J Biol Chem. 2000 Jan 28;275(4):2979-85. 10.1074/jbc.275.4.2979 PubMed 10644769
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