pNCMV
(Plasmid
#37453)
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Purpose(Empty Backbone)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 37453 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneCMV bam neo
- Backbone size (bp) 6550
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Modifications to backboneFlag/HA tag-containing linker added at BamHI site. Also added EcoRV site after tag.
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Vector typeMammalian Expression
- Promoter CMV
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Selectable markersNeomycin (select with G418)
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Tags
/ Fusion Proteins
- Flag (N terminal on backbone)
- HA (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV bam neo 5' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
There are a few discrepancies between the provided sequence and Addgene's quality control sequence, but they are not known to affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNCMV was a gift from Karl Munger (Addgene plasmid # 37453 ; http://n2t.net/addgene:37453 ; RRID:Addgene_37453) -
For your References section:
The human papillomavirus type 16 E6 oncoprotein activates mTORC1 signaling and increases protein synthesis. Spangle JM, Munger K. J Virol. 2010 Sep;84(18):9398-407. Epub 2010 Jul 14. 10.1128/JVI.00974-10 PubMed 20631133