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pAct5C-Hh
(Plasmid #37382)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37382 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAct5C
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hedgehog
  • Alt name
    hh
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    hh (a.k.a. Dmel_CG4637, CG4637, Dmel\CG4637, Dmhh, HH, Hg, Hh, Mir, Mrt, anon-WO0134654.19, anon-WO0182946.19, bar, bar-3, bar3, hg, l(3)hh, l(3)neo56, l(3)neo57)
  • Promoter actin5C

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer AC5
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Perrimon Lab plasmid #440

pAct5C-Hh construct was generated by PCR of full-length hh from a cDNA (gift from T. Kornberg; UCSF) with a 5' BamHI site and a 3' stop followed by a NotI site

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAct5C-Hh was a gift from Norbert Perrimon (Addgene plasmid # 37382 ; http://n2t.net/addgene:37382 ; RRID:Addgene_37382)

  • For your References section:

    A genome-wide RNA interference screen in Drosophila melanogaster cells for new components of the Hh signaling pathway. Nybakken K, Vokes SA, Lin TY, McMahon AP, Perrimon N. Nat Genet. 2005 Dec;37(12):1323-32. Epub 2005 Nov 20. 10.1038/ng1682 PubMed 16311596
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