pfast NPC1L1(NTD)
(Plasmid
#37366)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 37366 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFASTBAC
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Backbone manufacturerinvitrogen
- Backbone size w/o insert (bp) 5000
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namenpc1l1
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SpeciesH. sapiens (human)
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Insert Size (bp)800
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Mutationaa22-284only
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Entrez GeneNPC1L1 (a.k.a. LDLCQ7, NPC11L1, SLC65A2)
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Tag
/ Fusion Protein
- His (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer polyhedrin-fwd
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
There is a TEV cleavage site downstream of His tag.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pfast NPC1L1(NTD) was a gift from Johann Deisenhofer (Addgene plasmid # 37366 ; http://n2t.net/addgene:37366 ; RRID:Addgene_37366) -
For your References section:
The structure of the NPC1L1 N-terminal domain in a closed conformation. Kwon HJ, Palnitkar M, Deisenhofer J. PLoS One. 2011 Apr 15;6(4):e18722. 10.1371/journal.pone.0018722 PubMed 21525977