pBAD-LSSmOrange
(Plasmid #37129)

• Depositing Lab: Vladislav Verkhusha
• Publication: Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

Backbone

• Vector backbone: pBAD/His-B
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 3999
• Total vector size (bp): 4710
• Modifications to backbone: Several amino acids between His-tag and LSSmOrange were removed.
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: LSSmOrange
• Insert Size (bp): 710
• Mutation: A44V/F84L/W145M/I165D/M167L/G202D compared to mOrange (but numbering relative to EGFP)
• Promoter: araB
• Tag / Fusion Protein:
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pBAD-fwd
• 3′ sequencing primer: pBAD-rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry