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Addgene

pCMV-GLI(-)TAD
(Plasmid #37113)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37113 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 7700
  • Modifications to backbone
    Inserted GLI cDNA with a 1,409-bp AccI fragment deleted from the 3' end into the HindIII/XbaI site of pcDNA3.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GLI1
  • Species
    H. sapiens (human)
  • Mutation
    deleted 1,409 bp AccI fragment from 3' end of GLI cDNA
  • Entrez Gene
    GLI1 (a.k.a. GLI, PAPA8, PPD1)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-
  • 3′ sequencing primer ---
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-GLI(-)TAD was a gift from David Walterhouse (Addgene plasmid # 37113 ; http://n2t.net/addgene:37113 ; RRID:Addgene_37113)

  • For your References section:

    GLI activates transcription through a herpes simplex viral protein 16-like activation domain. Yoon JW, Liu CZ, Yang JT, Swart R, Iannaccone P, Walterhouse D. J Biol Chem. 1998 Feb 6;273(6):3496-501. 10.1074/jbc.273.6.3496 PubMed 9452474
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