pBabe-puro-Smad4-Flag
(Plasmid #37041)

• Depositing Lab: Sam Thiagalingam
• Publication: Papageorgis et al Cancer Res. 2011 Feb 1;71(3):998-1008. Epub 2011 Jan 18.

Backbone

• Vector backbone: pBabe-puro
• Backbone manufacturer: Weinberg Lab
• Backbone size w/o insert (bp): 5169
• Total vector size (bp): 7000
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Smad4
• Species: H. sapiens (human)
• Insert Size (bp): 1700
• Entrez Gene: SMAD4 (a.k.a. DPC4, JIP, MADH4, MYHRS)
• Promoter: 5'LTR
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SnaBI (destroyed during cloning)
• 3′ cloning site: SnaBI (destroyed during cloning)
• 5′ sequencing primer: pBABE5'
• 3′ sequencing primer: pBABE3'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Joan Massague, Memorial Sloan Kettering
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert was blunt-ended and ligated into SnaBI-digested pBabe-puro vector